By I. Silver (Eds.)

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This gain amounted to nearly four times the 0 time level. m. or below, however, bacterial counts for the ozone treatments could not be distinguished from controls. Similar results have been noted in an earlier study reported elsewhere (11). The bacterial loss or gain at four hours is summarized in Figure 1. F o r periods beyond four hours marked variability in the numbers of cultivable cells was noted in all groups save the higher ozone concentrations yielding mortalities 1 approaching 100 per cent.

In contrast, U R I outbreaks predominantly occurred during the refit period and, to a lesser extent, in the first two weeks at sea. It should be noted that one outbreak apparently occurred as late as the sixth week at sea; it is not known whether this patrol was earlier broken by a few days in harbor. Discussion These studies had three primary objectives. The first was to determine microbiological hazards that afford a substantial immediate threat to the mission and welfare of Polaris submarine crews.

Characteristics which were associated with increased U R I primarily fell into three groupings : 1. Factors associated with increased age and responsibilities, both in the service and at home (officers were excluded by insufficient numbers). 22 Proceedings Third International Symposium on Aerobiology 2. A subjective impression or history of recent or substantial U R I difficulties. 3. Bunk location on patrol: crew members occupying one group of 17 bunks had significantly more U R I , not alone in Patrols V through VIII, but also in two other submarines studied in Patrols II and XI.

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